Van Gerwen had previously only lost to Michael Smith, Jeffrey de Zwaan and Peter Wright this year but Wattimena who's performances on the Pro Tour have threatened this has ended 'Mighty Mike's Players Championship Streak.
Rob Cross remains the highest ranked player left in today and will now be favourite for the title. He though only just scraped through against Mickey Mansell 6-5.
Other big names through include Dave Chisnall, Jelle Klaasen, Mervyn King and James Wade all of which have not been in the best form but have been finding it again as of late.
Ryan Joyce continues his brilliant start to his PDC career by knocking out Robert Thornton 6-1 and Josh Payne has continued a great day by knocking out yesterday's finalist Chris Dobey 6-2.
Click here for more information about the Pro Tour and the prize fund breakdown.
Last 32
Jermaine Wattimena 6 | 4 Michael van Gerwen
Stephen Bunting 6 | 1 Jimmy Hendriks
Dave Chisnall 6 | 3 Cristo Reyes
Ricky Evans 6 | 4 Simon Stevenson
James Wilson 6 | 4 Daryl Gurney
Jelle Klaasen 6 | 4 Steve Beaton
Rob Cross 6 | 5 Mickey Mansell
Josh Payne 6 | 2 Chris Dobey
George Killington 6 | 5 Jan Dekker
Mervyn King 6 | 3 Kyle Anderson
Richard North 6 | 1 Steve West
Dimitri Van den Bergh 6 | 5 Kim Huybrechts
James Wade 6 | 5 Jeffrey de Zwaan
Ian White 6 | 3 Keegan Brown
Ryan Joyce 6 | 1 Robert Thornton
John Henderson 6 | 5 Madars Razma